Evaluating the agronomic efficiency of alternative phosphorus sources applied in Brazilian tropical soils.

Understanding the efficacy of alternative phosphorus (P) sources in tropical soils is crucial for sustainable farming, addressing resource constraints, mitigating environmental impact, improving crop productivity, and optimizing soil-specific solutions. While the topic holds great importance, current literature falls short in providing thorough, region-specific studies on the effectiveness of alternative P sources in Brazilian tropical soils for maize cultivation. Our aim was to assess the agronomic efficiency of alternative P sources concerning maize crop (Zea mays L.) attributes, including height, shoot dry weight, stem diameter, and nutrient accumulation, across five Brazilian tropical soils. In greenhouse conditions, we carried out a randomized complete block design, investigating two factors (soil type and P sources), evaluating five tropical soils with varying clay contents and three alternative sources of P, as well as a commercial source and a control group. We evaluated maize crop attributes such as height, dry weight biomass, and nutrient accumulation, P availability and agronomic efficiency. Our results showed that, although triple superphosphate (TSP) exhibited greater values than alternative P sources (precipitated phosphorus 1, precipitated phosphorus 2 and reactive phosphate) for maize crop attributes (e.g., height, stem diameter, shoot dry weight and phosphorus, nitrogen, sulfur, calcium and magnesium accumulation). For instance, PP1 source increased nutrient accumulation for phosphorus (P), nitrogen (N), and sulfur (S) by 37.05% and 75.98% (P), 34.39% and 72.07% (N), and 41.94% and 72.69% (S) in comparison to PP2 and RP, respectively. Additionally, PP1 substantially increased P availability in soils with high clay contents 15 days after planting (DAP), showing increases of 61.90%, 99.04%, and 38.09% greater than PP2, RP, and TSP. For Ca and Mg accumulation, the highest values were found in the COxisol2 soil when PP2 was applied, Ca = 44.31% and 69.48%; and Mg = 46.23 and 75.79%, greater than PP1 and RP, respectively. Finally, the highest values for relative agronomic efficiency were observed in COxisol2 when PP1 was applied. The precipitated phosphate sources (PP1 and PP2) exhibited a similar behavior to that of the commercial source (TSP), suggesting their potential use to reduce reliance on TSP fertilization, especially in soils with low clay contents. This study emphasized strategies for soil P management, aimed at assisting farmers in enhancing maize crop productivity while simultaneously addressing the effectiveness of alternative P sources of reduced costs.

Enrichment of medium-quality colostrum by adding colostrum replacer, combined or not with transition milk in the feeding of dairy calves.

Fifty Holstein calves were allocated in randomized blocks and distributed in a 2 × 2 factorial arrangement; (A) two sources of Ig: (1) Control: bovine colostrum (25% Brix); (2) Enriched colostrum: mid-quality bovine colostrum (20% Brix) enriched with colostrum replacer to 25% Brix; and (B) two transition feeding diets: (1) Whole milk (WM): supply of 4 L/day of whole milk for 3 days after the colostrum feeding; and (2) Formulated transition milk (FTM): supply 4 L/day of whole milk enriched with 70 g/L of colostrum replacer for 3 days after the colostrum feeding. Blood samples were collected at 0, 24, 48, and 72 h of age to determine total serum protein (TSP), glucose, non-esterified fatty acids (NEFA), erythrocyte and leukocyte concentrations. IgG was measured at 48 h. During the preweaning period, calves received 6 L/day of whole milk. Blood samples were collected weekly to determine TSP, glucose, and lactate. The colostrum protocols were equally efficient for transfer of passive immunity with IgG concentration at 48 h ≥ 49.6 g/L. Colostrum or transition feeding program did not influence the erythrocyte and leukocyte concentrations. The TSP concentration measured until 72 h was higher for calves fed maternal colostrum. Calves fed milk in the transition period had higher glucose concentrations. Calves receiving bovine colostrum and FTM had higher glucose concentrations in the preweaning period, while the enriched colostrum decreased plasma lactate concentrations. In summary, enrichment of mid-quality colostrum is an alternative in situations of a shortage of high-quality colostrum; however, feeding 4 L/day of FTM only for 3 days after colostrum feeding does not show additional benefits.

Essential oil blend supplementation in the milk replacer of dairy calves: Performance and health.

Supplementation of dairy calves with essential oils in the liquid diet can reduce the incidence and severity of infectious diseases and promote better performance. Our objective was to evaluate an essential oil blend containing peppermint, eucalyptus, and menthol crystals and its effect on performance and health during the pre and postweaning period of dairy calves. At birth, 40 dairy calves (34 males and 6 females) were blocked according to sex and birth weight, randomly assigned to one of two treatments-commercial milk replacer diluted at 14% (MR) and the same liquid diet plus essential oil blend (MREO) at a dose of 4 mL per calf per day, divided into two meals during the first 28 days of life. Calves were individually housed and fed 6 L/d of the liquid diet, divided into two meals, and received starter concentrate and water ad libitum. Weaning was gradually reduced by 1L per day at d 51 until complete weaning at 56 days. After weaning, calves were evaluated until 70 days of age, when the experimental period ended. Intake, fecal, and health scores were evaluated daily, weight and body measures were recorded weekly, and blood parameters were evaluated at weeks 1, 2, 3, 4, and 8. Calves fed MREO tended to have higher total dry matter intake during the preweaning period. Furthermore, MREO calves had lower health scores and fewer days with a health score ≥ 4 (suggestive of bronchopneumonia), tended to lower coughing days and fewer veterinary interventions preweaning, and tended to lower health scores postweaning. The supplementation with a blend of essential oils containing peppermint, eucalyptus, and menthol crystals can reduce respiratory problems. Further studies are needed to investigate the dose and the supplementation period.

Antioxidant, anti-inflammatory and analgesic activity of Mimosa acutistipula (Mart.) Benth.

ETHNOPHARMACOLOGICAL RELEVANCE: Medicinal plants belonging to the genus Mimosa, such as Mimosa tenuiflora, M. caesalpinifolia, and M. verrucosa are known for their popular use for asthma, bronchitis and fever. Ethnopharmacological studies report that Mimosa acutistipula is used to treat alopecia and pharyngitis, conditions that can be related to oxidative stress, inflammatory processes and painful limitations. However, there is no studies on its efficacy and mechanism of action. AIM OF THE STUDY: To elucidate the antioxidant, anti-inflammatory, analgesic and antipyretic activity of M. acutistipula leaves. MATERIALS AND METHODS: Phytochemical profile of M. acutistipula extracts was evaluated by several reaction-specific methods. Secondary metabolites such as tannins, phenols and flavonoids were quantified with colorimetric assays. In vitro antioxidant potential was evaluated using DPPH and ABTS + as free radical scavenging tests, FRAP and phosphomolybdenum as oxide-reduction assays, and anti-hemolytic for lipid peroxidation evaluation. In vivo anti-inflammatory evaluation was performed by paw edema, and peritonitis induced by carrageenan. Analgesic effect and its possible mechanisms were determined by acetic acid-induced abdominal writhing and the formalin test. Antipyretic activity was evaluated by yeast-induced fever. RESULTS: Cyclohexane, chloroform, ethyl acetate and methanol extracts of leaves had presence of tannins, flavonoids, phenol, alkaloids, terpenes (except methanolic extract), and saponins (only for methanolic and chloroformic extracts). In phenols, flavonoids and tannins quantification, methanolic and ethyl acetate extract had higher amounts of this phytocompounds. Ethyl acetate extract, due to its more expressive quantity of phenols and flavonoids, was chosen for carrying out the in vivo tests. Due to the relationship between oxidative stress and inflammation, antioxidant tests were performed, showing that ethyl acetate extract had a high total antioxidant activity (70.18%), moderate activity in DPPH radical scavenging, and a moderate ABTS + radical inhibition (33.61%), and FRAP assay (112.32 µg Fe2+/g). M. acutistipula showed anti-inflammatory activity, with 54.43% of reduction in paw edema (50 mg/kg) when compared to the vehicle. In peritonitis test, a reduction in the concentration of NO could be seen, which is highly involved in the anti-inflammatory activity and is responsible for the increase in permeability. In the analgesic evaluation, most significant results in writhing test were seen at 100 mg/kg, with a 34.7% reduction of writhing. A dual mechanism of action was confirmed with the formalin test, both neurogenic and inflammatory pain were reduced, with a mechanism via opioid route. In the antipyretic test, results were significantly decreased at all concentrations tested. CONCLUSION: M. acutistipula leaves ethyl acetate extract showed expressive concentrations of phenolic compounds and antioxidant activity. It also exhibited anti-inflammatory and analgesic activity, besides its antipyretic effect. Thus, these results provide information regarding its popular use and might help future therapeutics involving this specimen.

Intake, digestibility, ruminal parameters, and performance in lamb fed with increasing levels of red propolis extract.

The objective of this study was to examine the effect of increasing levels of red propolis extract (RPE) on the intake, digestibility, feeding behavior, rumen parameters, metabolic parameters, and performance of feedlot lambs. Thirty-five uncastrated male Santa Inês lambs with an initial weight of 17.08 ± 2.36 kg were used in a completely randomized design with five treatments (0, 7, 14, 21, or 28 mL RPE/animal/day). The animals were confined for 68 days. Red propolis extract induced a negative quadratic response (P < 0.05) in the intakes of dry matter, organic matter, crude protein, ether extract, neutral detergent fiber, non-fibrous carbohydrates, and metabolizable energy. The apparent digestibility coefficients of dry matter, organic matter, and neutral detergent fiber, as well as the rumen concentration of NH3-N, also responded quadratically (P < 0.05) to RPE. Feeding efficiency increased linearly (P < 0.05) with the inclusion of RPE, whereas rumination efficiency was maximum (P < 0.05) at the RPE level of 16 mL/day. Red propolis extract induced a linear response (P < 0.05) in serum total protein, albumin, creatinine, and gamma-glutamyl transpeptidase. There was a quadratic effect on final body weight and average daily gain with minimum values for inclusion of RPE of 12.89 mL/day and 10.93 mL/day respectively. Feed efficiency rose linearly (P < 0.05) with the increasing concentrations of RPE in the diet. The inclusion of 21 mL RPE/day (8.5 mg total flavonoids/mL) in the diet of feedlot lambs is recommended to reduce the rumen NH3-N production and increase the animals' performance.

Therapeutic ultrasound versus injection of local anesthetic in the treatment of women with chronic pelvic pain secondary to abdominal myofascial syndrome: a randomized clinical trial.

BACKGROUND: Chronic pelvic pain (CPP) is defined as recurrent or continuous pain in the lower abdomen or pelvis, either non-menstrual or noncyclical, lasting for at least 6 months. There is strong evidence that up to 85% of patients with CPP have serious dysfunctions of the musculoskeletal system, including abdominal myofascial pain syndrome (AMPS). AMPS is characterized by intense and deep abdominal pain, originating from hyperirritable trigger points, usually located within a musculoskeletal band or its lining fascia. In the literature, there are few studies that address AMPS. OBJECTIVES: To evaluate and compare the efficacy of therapeutic ultrasound (TUS) and injection of local anesthetic (IA) to improve pain in women with abdominal myofascial syndrome secondary to CPP. STUDY DESIGN: Randomized controlled clinical trial. SETTING: Tertiary University Hospital. MATERIALS AND METHODS: A randomized clinical trial was conducted, patients were allocated to two types of treatment: group TUS (n = 18), and group IA (n = 20). The instruments used for evaluation and reassessment were the Visual Analog Scale, Numerical Categorical Scale, McGill Pain Questionnaire, and SF-36 quality of life assessment questionnaire. They were evaluated before starting treatment, 1 week after the end of treatment, and at 1, 3, and 6 months. RESULTS: TUS and IA were effective in reducing clinical pain and improving quality of life through the variables analyzed among study participants. There was no significant difference between groups. LIMITATIONS: absence of blinding; exclusion of women with comorbidities and other causes of CPP, the absence of a placebo group, the difference between the number of sessions used for each technique, and the COVID-19. CONCLUSION: Treatment with TUS and IA were effective in reducing clinical pain and improving quality of life in women with AMPS secondary to CPP. TRAIL REGISTRATION: We declare that this clinical trial has been registered under the number [(ReBEC) no. RBR-39czsv] on 07/18/2018 in the Brazilian Registry of Clinical Trials.

Ingestive behavior of Girolando steers supplemented on tropical pastures.

In this study, we evaluated the feeding behavior of Girolando steers on Brachiaria brizantha cultivar Marandu pastures. Twenty-two animals with an average initial weight of 209.09 ± 8.18 kg were distributed in a completely randomized design in a 3 × 2 factorial arrangement (three seasons and two nutritional plans (NP)) with 11 replicates, as follows: rainy season 1 (NP1, mineral mixture ad libitum, and NP2, nitrogen/energy supplement [2 g.kg‒1 body weight [BW]]); dry season (NP1, nitrogen/energy supplement [1 g.kg‒1 BW], and NP2, nitrogen/energy supplement [2 g.kg‒1 BW]); and rainy season 2 (NP1, mineral mixture ad libitum, and NP2, nitrogen/energy supplement ([1 g.kg‒1 BW]). Total chewing time was longer in NP1 (566.44 vs 528.33 min.day‒1 in NP2) (p < 0.05). The grazing, idle, trough, and total chewing times were affected by the interaction between nutritional plans and seasons. The period expended grazing was longer for the NP1. The idle time was affected by the nutritional plans and was lower for the NP1. Feeding at the trough was not affected by the nutritional plans. The animals of the NP2 showed the highest feed efficiencies in DM and NDF (0.91 and 0.52 vs 0.75 and 0.45 in NP1, respectively). Rainy season 2 had the highest efficiencies. The feeding behavior changes according to the supplementation level. Nutritional plan 2 in the second rainy season presented the best results.

Photodynamic therapy of adenoid hypertrophy in acute rhinosinusitis.

A first step for microorganisms to reach the respiratory system and cause infectious disease is colonization in the nasopharynx. Humans inhale a bacterial load of up to 106 per cubic meter of air per day [1], including transient microorganisms between the upper and lower airways. This can lead to lung infections, amounting to billions of dollars in annual direct treatment costs in the United States, depending on the etiologic agent [2,3]. Curcumin has been described as a photosensitizer (PS) that, activated at 450 nm, is efficient against planktonic bacteria [4] and biofilms [5]. At the same time, effects on microbial interactions are commonly detected in the upper respiratory tract and should be considered for the treatment of adenoids [6]. We, therefore, propose in this study to optimize photodynamic therapy (PDT) conditions in vitro by simulating bacterial coinfection conditions in nasal cavities evaluated by the response surface method, where we can evaluate interactions of treatment variables. From this, the clinical case of the treatment of rhinosinusitis was carried out using PDT with nasal lighting. The absence of symptoms that characterize the disease was monitored and evaluated by the Kepler Meyer method. The study points out considerations that can be evaluated for the treatment to be a possibility of clinical indication in the control of rhinosinusitis.

The influence of protected fat in supplements on the performance and carcass characteristics of Nellore beef bulls in tropical pasture.

The finishing stages for uncastrated Nellore beef cattle on tropical pasture often produce high yielding carcasses with minimal fat cover. This study aimed to evaluate the performance and carcass characteristics of uncastrated Nellore beef bulls fed a diet containing rumen-protected fat during the rainy period for 210 days. Eighty Nellore beef bulls with 28 months of age, and an initial live weight of 416 kg ± 16 kg, were allocated into eight paddocks (7.0 ha), totaling 56 ha, at 10 animals/paddock, in a continuous-grazing system. The pasture characteristics, performance production, loin-eye area (cm2), subcutaneous fat thickness (mm), and rump fat thickness were evaluated. There was no effect (P > 0.05) on the average total weight gain (203.00 kg) and average daily gain (0.98 kg/day). On the other hand, the subcutaneous fat thickness and rump fat thickness were 42.8% and 49.5% greater, respectively, when the animals were fed a diet containing rumen-protected fat inclusion (P < 0.05). Supplementation with rumen-protected fat provided a thicker fat layer in the carcass of Nellore beef bulls grazing on tropical pasture during the rainy period.

Subacute and low-dose tributyltin exposure disturbs the mammalian hypothalamus-pituitary-thyroid axis in a sex-dependent manner.

Tributyltin (TBT) is an endocrine disruptor chemical (EDC) capable of altering the proper function of the hypothalamus-pituitary thyroid (HPT) axis. This study aimed to evaluate the subacute effects of TBT on the HPT axis of male and female rats. A dose of 100 ng/kg/day TBT was used in both sexes over a 15-day period, and the morphophysiology and gene expression of the HPT axis were assessed. TBT exposure increased the body weight in both sexes, while food efficiency increased - only in male rats. It was also possible to note alterations in the thyroid, with the presence of a stratified epithelium, cystic degeneration, and increased interstitial collagen deposition. A reduction in T3 and T4 levels was only observed in TBT male rats. A reduction in TSH levels was observed in TBT female rats. Evaluating mRNA expression, we observed a decrease in hepatic D1 and TRH mRNA levels in TBT female rats. An increase in D2 mRNA expression in the hypothalamus was observed in TBT male rats. Additionally, no significant changes in TRH or hepatic D1 mRNA expression in TBT male rats or in hypothalamic D1 and D2 mRNA expression in TBT female rats were observed. Thus, we can conclude that TBT has different toxicological effects on male and female rats by altering thyroid gland morphophysiology, leading to abnormal HPT axis function, and even at subacute and low doses, it may be involved in complex endocrine and metabolic disorders.

Evaluation of the treatment of fever, pain and inflammation with Indigofera suffruticosa Miller Leaves Aqueous Extract.

ETHNOPHARMACOLOGICAL RELEVANCE: Indigofera suffruticosa has reports of its popular use for analgesy in several cultures. Besides that, all parts of this plant are used for some medicinal outcome. The leaves are used in teas, decoctions, juices and included in baths for treating fever and inflammatory processes. AIM OF THE STUDY: To evaluate the anti-inflammatory activity mechanisms of I. suffruticosa leave aqueous extract (IsAE). MATERIALS AND METHODS: Phytochemical screening of IsAE was performed by thin layer chromatography. Total flavonoid content was determined and expressed by milligram of quercetin equivalent per gram of extract (mgEQ/g). 50% of the lethal dose that kills animals (LD50) was determined by acute toxicity in mice. Anti-inflammatory activity was evaluated through carrageenan-induced paw edema, peritonitis, and protein denaturation inhibition. Anti-nociceptive potential was evaluated by acetic acid-induced writhing and formalin tests. Antipyretic activity was assessed by yeast-induced fever. RESULTS: Phytochemical analysis revealed the presence of flavonoids and acid gallic in a quantity of 33.9 mg QE/g. Acute toxicity evaluation resulted in a LD50 of 3807.88 mg/kg. For carrageenan-induced paw edema test, IsAE in both doses (20 and 100 mg/kg) reduced the edema in 83.93%. IsAE reduced nitric oxide (NO) production and leucocytes migration to peritonitis inflammation site and at a concentration of 0.5 mg/mL showed also inhibition of protein denaturation similar to indomethacin in the same concentration. IsAE inhibited in 72.60% the number of contortions in writhing test. In formalin test, IsAE was also efficient, but showed results only in the second phase. In addition, the concentration of 100 mg/kg reduced fever significantly. CONCLUSIONS: IsAE proved to be anti-inflammatory, acting in different parts of the inflammation process, confirming its popular use.

Efeitos da auriculoterapia com sementes de mostarda na dor lombar crônica de profissionais de enfermagem / Efectos de la auriculoterapia con semillas de mostaza en el dolor lumbar crónico de los profesionales de enfermería / Effects of auriculotherapy with mustard seeds on chronic low back pain of nursing professionals

RESUMO Mais de 60% dos trabalhadores de enfermagem apresentam episódio de lombalgia durante um ano, provocando um impacto socioeconômico negativo, uma vez que é uma das maiores causas de faltas e afastamentos do trabalho. A auriculoterapia é uma técnica da acupuntura de baixo custo e não invasiva, que utiliza o pavilhão auricular como um microssistema do organismo humano mapeado por pontos que, estimulados, podem tratar diversas enfermidades. Sendo assim, o objetivo deste estudo foi verificar a eficácia da auriculoterapia com sementes de mostarda (Brassica juncea) na melhora da dor, na funcionalidade e na mobilidade lombar de profissionais técnicos e auxiliares de enfermagem do sexo feminino com dor lombar crônica. Foi realizado um ensaio clínico randomizado cego, dividido em dois grupos: um utilizou sementes de mostarda para acupressão nos pontos auriculares "Shen-Men", "Rim", "Simpático" e "Coluna Lombar" e o grupo placebo utilizou espuma de poliuretano de baixa densidade no lugar das sementes. Cada grupo realizou quatro sessões de auriculoterapia, uma vez por semana. Os grupos foram analisados por meio de termogramas infravermelhos e algometria por pressão para a dor, a medida dedo-chão para mobilidade e Questionário Roland-Morris para funcionalidade da coluna, com intervalo de confiança de 95%. A auriculoterapia com sementes de mostarda reduziu a temperatura média nos termogramas analisados em 0,8°C, bem como, aumentou o limiar de dor à pressão na coluna lombar das voluntárias em 0,4 Kgf, o que demonstra uma melhora significativa da dor lombar. Portanto, a auriculoterapia com sementes de mostarda mostrou-se eficaz na melhora da dor lombar.

RESUMEN Más del 60% de los trabajadores de enfermería tienen un episodio de dolor lumbar durante un año, causando un impacto socioeconómico negativo, ya que es una de las mayores causas de ausencias y despidos del trabajo. La auriculoterapia es una técnica de acupuntura de bajo costo y no invasiva que utiliza el pabellón auricular como un microsistema del organismo humano mapeado por puntos que, estimulados, pueden tratar diversas enfermedades. Así, el objetivo de este estudio fue verificar la eficacia de la auriculoterapia con semillas de mostaza (Brassica juncea) en la mejora del dolor, la funcionalidad y la movilidad lumbar de mujeres profesionales técnicas y auxiliares de enfermería con dolor lumbar crónico. Se realizó un ensayo clínico aleatorizado ciego, dividido en dos grupos: uno utilizó semillas de mostaza para acupresión en los puntos auriculares "Shen-Men", "Riñón", "Simpático" y "Columna Lumbar" y el grupo placebo utilizó espuma de poliuretano de baja densidad en lugar de las semillas. Cada grupo realizó cuatro sesiones de auriculoterapia una vez por semana. Los grupos se analizaron mediante termogramas infrarrojos y algometría de presión para el dolor, prueba de distancia dedos-suelo para la movilidad y Cuestionario Roland-Morris para la funcionalidad de la columna vertebral, con un intervalo de confianza del 95%. La auriculoterapia con semillas de mostaza redujo la temperatura promedio en los termogramas analizados en 0,8°C, así como aumentó el umbral de dolor a presión en la columna lumbar de las voluntarias en 0,4 Kgf, lo que demuestra una mejora significativa en el dolor lumbar. Por lo tanto, la auriculoterapia con semillas de mostaza ha demostrado ser eficaz para mejorar el dolor lumbar.

ABSTRACT More than 60% of nursing workers have an episode of low back pain for one year, causing a negative socioeconomic impact, as this is one of the major causes of absence from work. Auriculotherapy is a low-cost, non-invasive acupuncture technique that uses the auricular pavilion as a microsystem of the human organism mapped by points that when stimulated can treat several ailments. Thus, this study aims to verify the efficacy of mustard seed (Brassica juncea) auriculotherapy in alleviating pain, improving functionality and lumbar mobility of female technical professionals and nursing assistants with chronic low back pain. A blind randomized clinical trial was conducted with two groups: one group used mustard seeds for acupressure in the ear points "Shen-Men," "Kidney," "Sympathetic," and "Lumbar Spine"; the placebo group used low density polyurethane foam in place of seeds. Each group performed four auriculotherapy sessions, one per week. The groups were analyzed using infrared thermograms and pressure algometry for pain, finger-floor measurement for mobility and Roland-Morris Questionnaire for spine functionality, with a 95% confidence interval. Mustard seed auriculotherapy reduced the average temperature in the thermograms analyzed by 0.8 °C, as well as increased the pain threshold to lumbar spine pressure of the volunteers by 0.4 Kgf, which shows a significant improvement of low back pain. Therefore, mustard seed auriculotherapy was effective in alleviating low back pain.

Bioactive Compounds and Evaluation of Antioxidant, Cytotoxic and Cytoprotective Effects of Murici Pulp Extracts (Byrsonima crassifolia) Obtained by Supercritical Extraction in HepG2 Cells Treated with H2O2.

The use of clean technologies in the development of bioactive plant extracts has been encouraged, but it is necessary to verify the cytotoxicity and cytoprotection for food and pharmaceutical applications. Therefore, the objective of this work was to obtain the experimental data of the supercritical sequential extraction of murici pulp, to determine the main bioactive compounds obtained and to evaluate the possible cytotoxicity and cytoprotection of the extracts in models of HepG2 cells treated with H2O2. The murici pulp was subjected to sequential extraction with supercritical CO2 and CO2+ethanol, at 343.15 K, and 22, 32, and 49 MPa. Higher extraction yields were obtained at 49 MPa. The oil presented lutein (224.77 µg/g), oleic, palmitic, and linoleic, as the main fatty acids, and POLi (17.63%), POO (15.84%), PPO (13.63%), and LiOO (10.26%), as the main triglycerides. The ethanolic extract presented lutein (242.16 µg/g), phenolic compounds (20.63 mg GAE/g), and flavonoids (0.65 mg QE/g). The ethanolic extract showed greater antioxidant activity (122.61 and 17.14 µmol TE/g) than oil (43.48 and 6.04 µmol TE/g). Both extracts did not show cytotoxicity and only murici oil showed a cytoprotective effect. Despite this, the results qualify both extracts for food/pharmaceutical applications.

Kaolin, Ascophyllum nodosum and salicylic acid mitigate effects of summer stress improving hazelnut quality.

BACKGROUND: Various strategies are needed to mitigate the negative impact on or to increase fruit quality. The effect of spraying kaolin (K), Ascophyllum nodosum (An) and salicylic acid (SA), in trees with and without irrigation, on quality and sensorial attributes of hazelnut (Grada de Viseu cultivar) was investigated during two consecutive years (2016 and 2017) in a commercial orchard located in Moimenta da Beira, Portugal. RESULTS: The treatments affected positively the biometric parameters nut and kernel weight, length, width, thickness and volume as well as the vitamin E level, antioxidant activity and content of some individual phenolics, such as protocatechuic acid, gallocatechin, catechin and epicatechin. The levels of amino acids in hazelnut kernels decreased in all the assayed treatments, while the kernel colour and sensorial attributes were not affected by the treatments. Hazelnut physical properties (nut and kernels), chemical and phytochemical composition and antioxidant activities were positively related. CONCLUSIONS: The application of K, An and SA improved the hazelnut tree response to climate change, without compromising the hazelnut chemical and sensorial quality. Furthermore, due to the similar observations for the same treatments with and without irrigation, it can be stated that K, An and SA can be efficient and cost-effective tools to mitigate summer stress in rain-fed orchards. © 2020 Society of Chemical Industry.

Anti-inflammatory, hypoglycemic, hypolipidemic, and analgesic activities of Plinia cauliflora (Mart.) Kausel (Brazilian grape) epicarp.

ETHNOPHARMACOLOGICAL RELEVANCE: Plinia cauliflora (Mart.) Kausel, known in Brazil as jabuticaba or jaboticaba has been used by Brazilian native populations for medicinal purposes, including those related to inflammatory conditions, such as asthma, diarrhea, disorders in female genitourinary tract, and tonsillitis. Inflammation has emerged as a main factor for the oxidative stress, hyperglycemia, and dyslipidemia present in chronic noncommunicable diseases (NCDs). Such disturbances have been a leading cause of death worldwide for decades, despite significant efforts in developing new therapies. Therefore, strengthening the relevance of ethnobotanic approaches, as P. cauliflora has the potential to become a natural, native, and traditional product to prevent and treat inflammation-associated diseases more effectively for more people. AIM OF THE STUDY: Evaluate anti-inflammatory, hypoglycemic, hypolipidemic, and analgesic properties of hydroethanolic extract of P. cauliflora epicarps (PcE). MATERIALS AND METHODS: Phytochemical compound from the PcE were identified through HPLC-DAD-ESI-MSn analysis. Antioxidant activity was determined by measuring 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The anti-inflammatory potential was investigated by carrageenan-induced paw edema and peritonitis in mice. Analgesic effect was assessed, in mice, though hot plate test and acetic acid-induced abdominal writhing. Antidiabetic and hypolipidemic potential were evaluated using alloxan-induced diabetic mice. RESULTS: Tannins, phenolic acids, and their derivatives were the predominant phytochemicals found. Overall, PcE showed different properties related to the treatment of clinical conditions associated with chronic diseases as a potent antioxidant activity, demonstrating a radical scavenging action similar to gallic acid. PcE oral administration also significantly reduced inflammation induced by paw edema and partially blocked leukocyte migration. Moreover, PcE produced peripheral and central analgesic effects, as evaluated in the writhing model and hot plate tests. Treatment with PcE significantly improved glucose levels and lipid markers in diabetic mice. CONCLUSIONS: P. cauliflora fruits are rich sources of secondary metabolites, mainly tannins and phenolic acids with high biological potential, which can effectively contribute to the approach of preventing and controlling chronic NCDs.

Antioxidant and antibacterial compounds from feijoa leaf extracts obtained by pressurized liquid extraction and supercritical fluid extraction.

This work investigated the efficiency of pressurized liquid extraction (PLE) and supercritical fluid extraction with cosolvent (SFE) in obtaining feijoa leaf extracts with high antioxidant and antibacterial activities. PLE was performed in customized equipment with environmentally friendly solvents, at 40/80 °C, in dynamic and static mode, and SFE was carried out for 210 min at 30 MPa, 55 °C and 15% ethanol-water as cosolvent. PLE extract (80 °C/ethanol-water/dynamic) provided the highest yield, total phenolic content, and antioxidant activities, but it was not effective as antibacterial agent. In contrast, SFE extract exhibited effectiveness against S. aureus, E. coli, and S. typhimurium, with minimum inhibitory concentration values from 14,211 to 3,553 µg.mL-1. Finally, gallic acid, catechin and isoquercetin were the major phenolics identified by liquid chromatography. Our findings revealed that feijoa leaf extracts by PLE and SFE have remarkable bioactivity, presenting a great potential to be used as natural food additives.

Topical hydrogel containing Achyrocline satureioides oily extract (free and nanocapsule) has anti-inflammatory effects and thereby minimizes irritant contact dermatitis.

Inflammatory dermatoses are prevalent worldwide, with impacts on the quality of life of patients and their families. The aim of this study was to determine the anti-inflammatory effects of Achyrocline satureioides oily extracts and nanocapsules on the skin using a mouse model of irritant contact dermatitis induced by croton oil, and a skin inflammation model induced by ultraviolet B (UVB) radiation. The mice were treated with 15 mg/ear oily extract (HG-OLAS) or nanocapsules (HG-NCAS) of A. satureioides incorporated into Carbopol® 940 hydrogels. We found that HG-OLAS and HG-NCAS formulations reduced ear edema in croton oil-induced lesions with maximum inhibitions of 54±7% and 74±3%, respectively. HG-OLAS and HG-NCAS formulations decreased ear edema induced by UVB radiation (0.5 J/cm2), with maximum inhibitions of 68±6% and 76±2% compared to the UVB radiation group, respectively. HG-OLAS and HG-NCAS modulated myeloperoxidase (MPO) activity after croton oil induction. Furthermore, croton oil and UVB radiation for 6 and 24 h, respectively, stimulated polymorphonuclear cells infiltration. The topical treatments reduced inflammatory processes, as shown by histological analysis. Together, the data suggest that topical application of A. satureioides oily extracts and nanocapsules produced antiedematogenic and anti-inflammatory effects. They constitute a compelling alternative for treatment of skin injuries.

Ashi Acupuncture Versus Local Anesthetic Trigger Point Injections in the Treatment of Abdominal Myofascial Pain Syndrome: A Randomized Clinical Trial.

BACKGROUND: Chronic pelvic pain (CPP) is defined as recurrent or continuous pain in the lower abdomen or pelvis, non-menstrual or non-cyclic, lasting at least 6 months. There is strong evidence that up to 85% of patients with CPP have serious dysfunction of the musculoskeletal system, including abdominal myofascial syndrome (AMPS). AMPS is characterized as deep abdominal pain, originating from hyperirritable trigger points, usually located within a musculoskeletal range or its fascia of coating. In the literature, there are few studies that address AMPS. OBJECTIVE: This study aimed to compare the responses of ashi acupuncture treatment and local anesthetic injection in the treatment of chronic pelvic pain secondary to abdominal myofascial pain syndrome in women. STUDY DESIGN: Randomized controlled clinical trial. SETTING: Tertiary University Hospital. METHODS: Women with a clinical diagnosis of CPP secondary to AMPS were randomized and evaluated using instruments to assess clinical pain, namely, the visual analogue scale (VAS), numerical categorial scale (NCS), and the McGill Questionnaire, after receiving treatment with ashi acupuncture (group A, n = 16) or local anesthetic injections (group B, n = 19). They were reevaluated after one week and one, 3, and 6 months after each treatment, in addition to assessments of pain and adverse events performed during the sessions. RESULTS: Ashi acupuncture and local anesthetic injections were both effective in reducing clinical pain assessed through the analyzed variables among study participants. There was no difference between the groups and there was a strong correlation between these pain assessment instruments. LIMITATIONS: The absence of blinding to the different forms of treatment among the patients and the researcher directly involved in the treatment, the absence of a placebo group, the selective exclusion of women with comorbidities and other causes of CPP, and the difference between the number of sessions used for each technique. CONCLUSION: Treatments with ashi acupuncture and local anesthetic injections were effective in reducing clinical pain in women with abdominal myofascial pain syndrome.

Quality preservation of sweet cherry cv. 'staccato' by using glycine-betaine or Ascophyllum nodosum.

Pre-harvest application of exogenous compounds has been employed in many crops, as a cultural practice, to promote their adaptation to a new climate-changing environment. Effect of foliar pre-harvest application of salicylic acid, glycine-betaine complex and seaweed extract (Ascophyllum nodosum) on the cherry quality from 'Staccato' cultivar was studied. Treatments significantly affected (p < 0.01) the fruit size, soluble solids content, pH, colour, polyphenols, vitamin C and antioxidant activity. Glycine-betaine and A. nodosum treated cherries presented higher dimensions, soluble solids content and pH and lower acidity. In addition, these cherries had a higher content of polyphenols and vitamin C and antioxidant capacity, but lower values of L*, C*ab and hue angle, meaning that both treatments can rise the fruit ripening process. Therefore, the pre-harvest application of glycine-betaine and A. nodosum can be a good alternative to promote the adaptation of sweet cherry tree to stressful environmental conditions, without compromising the fruit quality.

Products Derived from Buchenavia tetraphylla Leaves Have In Vitro Antioxidant Activity and Protect Tenebrio molitor Larvae against Escherichia coli-Induced Injury.

The relevance of oxidative stress in the pathogenesis of several diseases (including inflammatory disorders) has traditionally led to the search for new sources of antioxidant compounds. In this work, we report the selection of fractions with high antioxidant action from B. tetraphylla (BT) leaf extracts. In vitro methods (DPPH and ABTS assays; determination of phenolic and flavonoid contents) were used to select products derived from B. tetraphylla with high antioxidant action. Then, the samples with the highest potentials were evaluated in a model of injury based on the inoculation of a lethal dose of heat-inactivated Escherichia coli in Tenebrio molitor larvae. Due to its higher antioxidant properties, the methanolic extract (BTME) was chosen to be fractionated using Sephadex LH-20 column-based chromatography. Two fractions from BTME (BTFC and BTFD) were the most active fractions. Pre-treatment with these fractions protected larvae of T. molitor from the stress induced by inoculation of heat-inactivated E. coli. Similarly, BTFC and BTFD increased the lifespan of larvae infected with a lethal dose of enteroaggregative E. coli 042. NMR data indicated the presence of aliphatic compounds (terpenes, fatty acids, carbohydrates) and aromatic compounds (phenolic compounds). These findings suggested that products derived from B. tetraphylla leaves are promising candidates for the development of antioxidant and anti-infective agents able to treat oxidative-related dysfunctions.